10.1126/science.abj4008 #530
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davidsebfischer
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* added data loader d10_1126_science_abj4008 #530 * minor CLI improvements * added validate to template checking
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* improved guidance through CLI pipeline through action messages and error messages * improved curation documentation * relabled export phase in summary * CLI: add automatic detection of container for path handling * CLI: adapt path checking to container usecase * CLI: make env variables usable also without containers * CLI: add automatic PR submission in container * fix template creation CLI run * switched data library, CLI and curation annotation to yaml version 1.2 (#528) * improved curation docs * improved guidance in sfaira create-dataloader * improved reaction to existing loaders * improved data loader linting in CLI * support for count and processed data matrices in same loader, deprecates "normalization" * support for feature type annotation, allows for ATAC and CITE data (feature_type) * support for based genetic modification meta data (gm) * support for basic treatment meda data (treatment) * support for cell tracking in meta studies (source_doi) * support for spatial data * support for VDJ data * support for velocity data * support for reference genome annotation * support for arbirtrary organisms in genome container * added phase Pe * Data/10.1126/science.abj4008 (#531) * d10_1126_science_abj4008 #530 * added validate to template checking * improvements to VDJ interface * d10_1126_science_abe6474 (#532) * fixed sfaira structure DOI input to annotate * annotated GEO look up * d10_1126_sciimmunol_abd1554 (#471) * d10_1126_sciimmunol_abd1554 * dno_doi_luecken neurips loader update (#472) * fixed rich printing in CLI * added documentation of compressed and r file reading * Fix d10_1016_j_cell_2021_01_053 to work with GEO (#469) * added validation statement at the end of finalize * d10_1038_s41591_020_1061_7 * updated required meta data section in docs * disabled default cellxgene collection meta data caching which resulted in bug when collection were updated on remote Co-authored-by: davidsebfischer <david.seb.fischer@gmail.com> Co-authored-by: le-ander <20015434+le-ander@users.noreply.github.com> Co-authored-by: Laura Martens <laura.d.martens@icloud.com> Co-authored-by: xlancelottx <33050110+xlancelottx@users.noreply.github.com>
davidsebfischer
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* fix NA to empty template values (#434) Signed-off-by: zethson <lukas.heumos@posteo.net> * added bug fix for subsetting + .X method of DistributedstoreAnndata (#431) * Feature/trainer test paths (#437) * made trainer paths more flexible * do not check md5 of weighs if None (#442) * added fix to reduce size of pickle files (#440) * Dataset/d10 1016 j cell 2020 08 001 (#402) Co-authored-by: davidsebfischer <david.seb.fischer@gmail.com> * improved dask array concatenatin for .X method (#444) * Rewrite test_store.py script (#436) * Feature/dno doi luecken (#439) * added data loader dno_doi_luecken * improved CLI and documentation * 10.1101/2021.07.19.452954 and data loader tsv update * d10_1101_2021_07_19_452954 (10.1101/2021.07.19.452954) dataloader (#333) * New ontology map tsv interface with one tsv per ontology (#449) * CLI commands for cache clearing and reloading (#449) * datal loader 10.1038/s41467-021-27619-4 (desribed in #450) * Issue template for data loader request * d10_1038_s41586_019_1631_3: updated annotation and moved to format 1.1 (#467) * Rewrite batch schedule classes (#448) * rewrote batch_schedule to work directly on the supplied indicies * Torch data adaptor (#433) * added torch.utils.data.IterableDataset and torch Dataset class * added adpator unit test * cached query of assembly name to ensembl ftp server file query was already cached, but assembly name retrieval based on release version was not cached * added random rounding in intercalated batch data laoder * updated test splitting * enabled store loading from multiple paths * deprecated optional dependency of Dataset attributes on meta data files * fixed d10_1038_s41586_019_1631_3 * relaxed required arguments for DistributedStoreAnndata * added a full data set batch schedule * feature / added method to persist dask.array of DaoStroage into memory + small bug fixes (#447) * dask.array of DaoStore can now be persisted into memory in CSR format * improved docstring documentation for .adataptor * extended store docs * refactored distributedstore to store and minor obs indexing and cart default fixes * fixed obs indexing bug with empty single cart within multi cart Co-authored-by: felix0097 <47145207+felix0097@users.noreply.github.com> * bug fix for move_to_memory method of CartDask class (#475) * D10 1038 s41467 020 15543 y (#462) * 10.1038/s41467-020-15543-y * Feature: Option to shuffle datasets before writing dao stores (#473) * datasets can be shuffled now before writing dao store * Feature: match zarr chunks for randomized_batch_schedule=True for CartDask (#476) * random_batch_schedule for DaskCart now matches dask partitions * rearanged imports + removed debugging assertion statement * Create dataloader (#468) * bug fix - added .compute for .x method in DaskCart (#479) * Feature: Save .obs data as categorical dtype (#478) * str obs data is now stored and processed as categorical * fixed check for categorical dtype * Bug-Fix: d10_1038_s41467_021_27619_4 DataLoader (#480) * put column 3 back into var dataframe and named column feature_class * fixed gtf interface for newer ensembl releases for genes without symbol (#484) * add wheel to python build CI to avoid legacy setup.py install (#493) * Feature: add shuffle buffer to DaskCart (#474) * Refactor estimator code to support estimators for torch models (#485) * refactored estimator code into tf.keras code and base estimator code. this prepares side-by-side keras and torch code. * added torch losses and metrics * Improvements to CLI usability (#452) * improved guidance through CLI pipeline through action messages and error messages * improved curation documentation * relabled export phase in summary * CLI: add automatic detection of container for path handling * CLI: adapt path checking to container usecase * CLI: make env variables usable also without containers * CLI: add automatic PR submission in container * fix template creation CLI run * switched data library, CLI and curation annotation to yaml version 1.2 (#528) * improved curation docs * improved guidance in sfaira create-dataloader * improved reaction to existing loaders * improved data loader linting in CLI * support for count and processed data matrices in same loader, deprecates "normalization" * support for feature type annotation, allows for ATAC and CITE data (feature_type) * support for based genetic modification meta data (gm) * support for basic treatment meda data (treatment) * support for cell tracking in meta studies (source_doi) * support for spatial data * support for VDJ data * support for velocity data * support for reference genome annotation * support for arbirtrary organisms in genome container * added phase Pe * Data/10.1126/science.abj4008 (#531) * d10_1126_science_abj4008 #530 * added validate to template checking * improvements to VDJ interface * d10_1126_science_abe6474 (#532) * fixed sfaira structure DOI input to annotate * annotated GEO look up * d10_1126_sciimmunol_abd1554 (#471) * d10_1126_sciimmunol_abd1554 * dno_doi_luecken neurips loader update (#472) * fixed rich printing in CLI * added documentation of compressed and r file reading * Fix d10_1016_j_cell_2021_01_053 to work with GEO (#469) * added validation statement at the end of finalize * d10_1038_s41591_020_1061_7 * updated required meta data section in docs * disabled default cellxgene collection meta data caching which resulted in bug when collection were updated on remote Co-authored-by: davidsebfischer <david.seb.fischer@gmail.com> Co-authored-by: le-ander <20015434+le-ander@users.noreply.github.com> Co-authored-by: Laura Martens <laura.d.martens@icloud.com> Co-authored-by: xlancelottx <33050110+xlancelottx@users.noreply.github.com> * added new figures (#538) * added new figures * activated cellxgene caching for unit tests (#539) Co-authored-by: Leander <20015434+le-ander@users.noreply.github.com> Co-authored-by: Lukas Heumos <lukas.heumos@posteo.net> Co-authored-by: felix0097 <47145207+felix0097@users.noreply.github.com> Co-authored-by: Karin Hrovatin <47607471+Hrovatin@users.noreply.github.com> Co-authored-by: soerenab <36963673+soerenab@users.noreply.github.com> Co-authored-by: Laura Martens <laura.d.martens@icloud.com> Co-authored-by: xlancelottx <33050110+xlancelottx@users.noreply.github.com>
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CRISPR activation and interference screens decodestimulation responses in primary human T cells
Ralf Schmidt
https://www.science.org/doi/10.1126/science.abj4008
data: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE190604
assay: 10x 3' v3
GRCh38-2020-A > https://support.10xgenomics.com/single-cell-gene-expression/software/release-notes/build#GRCh38_2020A: Homo_sapiens.GRCh38.98
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