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✨ Add liftover and collapse workflow #337

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merged 15 commits into from
Apr 30, 2023

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migbro
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@migbro migbro commented Mar 10, 2023

Purpose/implementation Section

What scientific question is your analysis addressing?

How to make older RNA expression datasets compatible with new ones

What was your approach?

Lifttover using a GTF, leave genes alone that weren't in the GTF, collapse repeat symbols on highest row mean

What GitHub issue does your pull request address?

d3b-center/ticket-tracker-OPC#521
and
d3b-center/ticket-tracker-OPC#522

Directions for reviewers. Tell potential reviewers what kind of feedback you are soliciting.

Which areas should receive a particularly close look?

You can use this task https://cavatica.sbgenomics.com/u/brownm28/playground/tasks/8df870cc-69b0-4b44-a55e-205af9c7c874/ to see an example.

Is there anything that you want to discuss further?

In practice, use the gencode 39 gtf, and name the output file appropriately. Do I need to do something with circleci?

Is the analysis in a mature enough form that the resulting figure(s) and/or table(s) are ready for review?

Yes - readme helps as well

Results

What types of results are included (e.g., table, figure)?

Outputs

What is your summary of the results?

They look great, and line count using v36 as a gold standard from task https://cavatica.sbgenomics.com/u/d3b-bixu-ops/open-target-tcga-rnaseq-counts/tasks/580834b3-cb6a-4ac1-b188-826aef4203dc/ is the same

Reproducibility Checklist

  • The dependencies required to run the code in this pull request have been added to the project Dockerfile.
  • This analysis has been added to continuous integration.

Documentation Checklist

  • This analysis module has a README and it is up to date.
  • This analysis is recorded in the table in analyses/README.md and the entry is up to date.
  • The analytical code is documented and contains comments.

@migbro migbro added documentation Improvements or additions to documentation enhancement New feature or request labels Mar 10, 2023
@migbro migbro self-assigned this Mar 10, 2023
📝 edited readme to reflect changes
➖ dropped superfluous shell script
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The CWL looks mostly good. Lots of comments on the python though.

migbro and others added 3 commits March 14, 2023 13:23
Co-authored-by: Dan Miller <dmiller15@users.noreply.github.com>
🔨 wf refactor
🏆 address PR concerns and suggestions
@migbro migbro requested a review from dmiller15 March 14, 2023 18:41
migbro and others added 2 commits March 14, 2023 15:26
Co-authored-by: Dan Miller <dmiller15@users.noreply.github.com>
@migbro migbro requested a review from dmiller15 March 14, 2023 19:40
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migbro commented Mar 14, 2023

Got an updated task: https://cavatica.sbgenomics.com/u/brownm28/playground/tasks/2990d1eb-57d3-4fce-9be2-33f026049e1d/#
I think we should be good now

Co-authored-by: Dan Miller <dmiller15@users.noreply.github.com>
@migbro migbro requested a review from dmiller15 March 14, 2023 19:43
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Code looks much better! Nice work 👍

@jharenza
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Merging without GA, since this is not being run in GA.

@jharenza jharenza merged commit a7170f2 into d3b-center:dev Apr 30, 2023
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3 participants