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Importing data using BactMAP

Renske van Raaphorst edited this page Oct 10, 2019 · 4 revisions

Data from segmentation programs

program detects function
Oufti
  • cell meshes
  • spots
  • objects
  • genealogy
extr_Oufti()
SuperSegger
  • cell meshes
  • spots
  • genealogy
extr_SuperSeggerClist and extr_SuperSeggerCells
ObjectJ
  • cell bounding box
extr_ObjectJ()
Morphometrics
  • cell meshes
  • objects
extr_Morphometrics()
MicrobeJ
  • cell meshes
  • spots
  • objects
extr_MicrobeJ()
Custom import from other program
  • cell meshes
  • spots
  • objects
extr_Meshes(), extr_Spots()

Data from spot detection

Now, only the peak fitter and tracker output data from ISBatch are standardly importable in BactMAP. Other spot tracking output can be imported from .CSV or .TXT using extr_Spots.

program function:
ISBatch extr_ISBatch()
custom import from other program extr_Spots()

TIFF data

Uploading the raw tiff stacks can be useful for making kymographs or projections without doing spot or object detection first, or to check your segmentation/detection visually.

data type function:
greyscale, single channel TIFF/TIF image or image stack extr_OriginalStack
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