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[ERROR]Task Node(0-rawreads/build) failed with exit-code=1 using SLURM #109

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NicMAlexandre opened this issue Mar 13, 2019 · 17 comments
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@NicMAlexandre
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NicMAlexandre commented Mar 13, 2019

Job script:

#!/bin/bash

#SBATCH -p LM
#SBATCH -t 336:00:00
#SBATCH -N 1
#SBATCH --mem=200GB

# run it!
fc_run fc_run.cfg

Config file

[General]
input_fofn = fasta.fofn
input_type = raw
pa_DBdust_option=true
pa_fasta_filter_option=streamed-median

pa_DBsplit_option = -a -x500 -s200
ovlp_DBsplit_option =-x500 -s200

pa_HPCTANmask_option=
pa_REPmask_code=0,300;0,300;0,300

genome_size=1100000000
seed_coverage=30
length_cutoff=-1
pa_HPCdaligner_option=-v -B128 -M24
pa_daligner_option=-e0.8 -l2000 -k18 -h480  -w8 -s100
falcon_sense_option=--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400
falcon_sense_greedy=False

ovlp_daligner_option=-e.96 -s1000 -h60
ovlp_HPCdaligner_option=-v -M24 -l500

overlap_filtering_setting=--max-diff 100 --max-cov 100 --min-cov 2
fc_ovlp_to_graph_option=
length_cutoff_pr=1000

[job.defaults]
job_type=slurm
pwatcher_type=blocking
JOB_QUEUE = default
MB = 32768
NPROC = 6
njobs = 32
submit = srun --wait=0 -p myqueue -J ${JOB_NAME} -o ${JOB_STDOUT} -e ${JOB_STDERR} --mem-per-cpu=${MB}M --cpus-per-task=${NPROC} ${JOB_SCRIPT}

Error

falcon-kit 1.2.6
pypeflow 2.1.1
[INFO]Setup logging from file "None".
[INFO]$ lfs setstripe -c 12 /pylon5/mc5fqip/orca21 >
[WARNING]'lfs setstripe -c 12 /pylon5/mc5fqip/orca21' failed to produce any output.
[INFO]Lustre filesystem detected. This lfs stripe (12) should propagate to subdirs of '/pylon5/mc5fqip/orca21'.
[INFO]fc_run started with configuration fc_run.cfg
[INFO]cfg=
{
  "General": {
    "LA4Falcon_preload": false,
    "avoid_text_file_busy": true,
    "bestn": 12,
    "dazcon": false,
    "falcon_sense_greedy": false,
    "falcon_sense_option": "--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400",
    "falcon_sense_skip_contained": false,
    "fc_ovlp_to_graph_option": " --min-len 1000",
    "genome_size": "1100000000",
    "input_fofn": "fasta.fofn",
    "input_type": "raw",
    "length_cutoff": "-1",
    "length_cutoff_pr": "1000",
    "overlap_filtering_setting": "--max-diff 100 --max-cov 100 --min-cov 2",
    "ovlp_DBdust_option": "",
    "ovlp_DBsplit_option": "-x500 -s200",
    "ovlp_HPCdaligner_option": "-v -M24 -l500",
    "ovlp_daligner_option": "-e.96 -s1000 -h60",
    "pa_DBdust_option": "true",
    "pa_DBsplit_option": "-a -x500 -s200",
    "pa_HPCTANmask_option": "",
    "pa_HPCdaligner_option": "-v -B128 -M24",
    "pa_REPmask_code": "0,300;0,300;0,300",
    "pa_daligner_option": "-e0.8 -l2000 -k18 -h480  -w8 -s100",
    "pa_dazcon_option": "-j 4 -x -l 500",
    "pa_fasta_filter_option": "streamed-median",
    "pa_subsample_coverage": 0,
    "pa_subsample_random_seed": 12345,
    "pa_subsample_strategy": "random",
    "seed_coverage": "30",
    "skip_checks": false,
    "target": "assembly"
  },
  "job.defaults": {
    "JOB_QUEUE": "default",
    "MB": "32768",
    "NPROC": "6",
    "job_type": "slurm",
    "njobs": "32",
    "pwatcher_type": "blocking",
    "submit": "srun --wait=0 -p myqueue -J ${JOB_NAME} -o ${JOB_STDOUT} -e ${JOB_STDERR} --mem-per-cpu=${MB}M --cpus-per-task=${NPROC} ${JOB_SCRIPT}",
    "use_tmpdir": false
  },
  "job.step.asm": {},
  "job.step.cns": {},
  "job.step.da": {},
  "job.step.dust": {},
  "job.step.la": {},
  "job.step.pda": {},
  "job.step.pla": {}
}
[INFO]In simple_pwatcher_bridge, pwatcher_impl=<module 'pwatcher.blocking' from '/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pwatcher/blocking.pyc'>
[INFO]job_type='slurm', (default)job_defaults={'JOB_QUEUE': 'default', 'pwatcher_type': 'blocking', 'use_tmpdir': False, 'MB': '32768', 'job_type': 'slurm', 'submit': 'srun --wait=0 -p myqueue -J ${JOB_NAME} -o ${JOB_STDOUT} -e ${JOB_STDERR} --mem-per-cpu=${MB}M --cpus-per-task=${NPROC} ${JOB_SCRIPT}', 'NPROC': '6', 'njobs': '32'}, use_tmpdir=False, squash=False, job_name_style=0
[INFO]Setting max_jobs to 32; was None
[INFO]Num unsatisfied: 2, graph: 2
[INFO]About to submit: Node(0-rawreads/build)
[INFO]Popen: 'srun --wait=0 -p myqueue -J P2c7b8ae23d7f98 -o /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash.stdout -e /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash.stderr --mem-per-cpu=4000M --cpus-per-task=1 /pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pwatcher/mains/job_start.sh'
[INFO](slept for another 0.0s -- another 1 loop iterations)
[INFO](slept for another 0.3s -- another 2 loop iterations)
[INFO](slept for another 1.2s -- another 3 loop iterations)
[INFO](slept for another 3.0s -- another 4 loop iterations)
[INFO](slept for another 6.0s -- another 5 loop iterations)
[INFO](slept for another 10.5s -- another 6 loop iterations)
[INFO](slept for another 16.8s -- another 7 loop iterations)
[INFO](slept for another 25.2s -- another 8 loop iterations)
[INFO](slept for another 36.0s -- another 9 loop iterations)
srun: error: l006: task 4: Exited with exit code 1
srun: error: l006: task 2: Exited with exit code 1
[INFO](slept for another 49.5s -- another 10 loop iterations)
[INFO](slept for another 66.0s -- another 11 loop iterations)
[INFO](slept for another 85.8s -- another 12 loop iterations)
srun: error: l006: tasks 0,3: Exited with exit code 1
[INFO](slept for another 109.2s -- another 13 loop iterations)
[INFO](slept for another 135.6s -- another 14 loop iterations)
[INFO](slept for another 150.0s -- another 15 loop iterations)
[INFO](slept for another 160.0s -- another 16 loop iterations)
[INFO](slept for another 170.0s -- another 17 loop iterations)
[INFO](slept for another 180.0s -- another 18 loop iterations)
[INFO](slept for another 190.0s -- another 19 loop iterations)
[INFO](slept for another 200.0s -- another 20 loop iterations)
[INFO](slept for another 210.0s -- another 21 loop iterations)
srun: error: l006: task 1: Exited with exit code 1
[ERROR]Task Node(0-rawreads/build) failed with exit-code=1
[ERROR]Some tasks are recently_done but not satisfied: set([Node(0-rawreads/build)])
[ERROR]ready: set([])
	submitted: set([])
[ERROR]Noop. We cannot kill blocked threads. Hopefully, everything will die on SIGTERM.
Traceback (most recent call last):
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin/fc_run", line 11, in <module>
    load_entry_point('falcon-kit==1.2.6', 'console_scripts', 'fc_run')()
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 726, in main
    main1(argv[0], args.config, args.logger)
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 76, in main1
    input_fofn_fn=input_fofn_fn,
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 242, in run
    dist=Dist(NPROC=4, MB=4000, job_dict=config['job.step.da']),
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/pype.py", line 106, in gen_parallel_tasks
    wf.refreshTargets()
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/simple_pwatcher_bridge.py", line 278, in refreshTargets
    self._refreshTargets(updateFreq, exitOnFailure)
  File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/simple_pwatcher_bridge.py", line 362, in _refreshTargets
    raise Exception(msg)
Exception: Some tasks are recently_done but not satisfied: set([Node(0-rawreads/build)])

Conda list

# packages in environment at /pylon5/mc5fqip/orca21/anaconda3/envs/myenv:
#
# Name                    Version                   Build  Channel
asn1crypto                0.24.0                   py27_0  
avro-python2              1.8.2                      py_1    bioconda
bcftools                  1.9                  h47928c2_2    bioconda
blas                      1.0                         mkl  
blasr                     5.3.2                hac9d22c_4    bioconda
blasr_libcpp              5.3.1                hac9d22c_3    bioconda
bzip2                     1.0.6                h14c3975_5  
ca-certificates           2019.1.23                     0  
certifi                   2019.3.9                 py27_0  
cffi                      1.12.1           py27h2e261b9_0  
chardet                   3.0.4                    py27_1  
cryptography              2.5              py27h1ba5d50_0  
curl                      7.63.0            hbc83047_1000  
cython                    0.29.5           py27he6710b0_0  
decorator                 4.3.2                    py27_0  
enum34                    1.1.6                    py27_1  
falcon-kit                1.2.6                    pypi_0    pypi
falcon-phase              1.0.0                    pypi_0    pypi
falcon-unzip              1.1.7                    pypi_0    pypi
future                    0.17.1                   py27_0  
genomicconsensus          2.3.2                    py27_3    bioconda
h5py                      2.8.0            py27h989c5e5_3  
hdf5                      1.10.2               hba1933b_1  
htslib                    1.9                  h47928c2_5    bioconda
idna                      2.8                      py27_0  
igvtools                  2.3.93                        0    bioconda
intel-openmp              2019.1                      144  
ipaddress                 1.0.22                   py27_0  
iso8601                   0.1.12                   py27_1  
krb5                      1.16.1               h173b8e3_7  
libcurl                   7.63.0            h20c2e04_1000  
libdeflate                1.0                  h14c3975_1    bioconda
libedit                   3.1.20181209         hc058e9b_0  
libffi                    3.2.1                hd88cf55_4  
libgcc-ng                 8.2.0                hdf63c60_1  
libgfortran-ng            7.3.0                hdf63c60_0  
libssh2                   1.8.0                h1ba5d50_4  
libstdcxx-ng              8.2.0                hdf63c60_1  
linecache2                1.0.0                    py27_0  
mkl                       2019.1                      144  
mkl_fft                   1.0.10           py27ha843d7b_0  
mkl_random                1.0.2            py27hd81dba3_0  
ncurses                   6.1                  he6710b0_1  
networkx                  2.2                      py27_1  
numpy                     1.16.2           py27h7e9f1db_0  
numpy-base                1.16.2           py27hde5b4d6_0  
openjdk                   8.0.152              h46b5887_1  
openssl                   1.1.1b               h7b6447c_1  
pb-dazzler                0.0.0                h470a237_0    bioconda
pb-falcon                 0.2.6            py27hc0aa232_3    bioconda
pbalign                   0.3.2                    py27_0    bioconda
pbbam                     0.19.0               h6678c95_1    bioconda
pbcommand                 1.1.1                    py27_2    bioconda
pbcore                    1.6.5                    py27_0    bioconda
pip                       19.0.3                   py27_0  
pycparser                 2.19                     py27_0  
pyopenssl                 19.0.0                   py27_0  
pysam                     0.15.2           py27h1671916_1    bioconda
pysocks                   1.6.8                    py27_0  
python                    2.7.15               h9bab390_6  
python-consensuscore      1.1.1            py27h3f2a3d2_3    bioconda
python-consensuscore2     3.4.1                    py27_0    bioconda
python-edlib              1.2.3.post1      py27h470a237_0    bioconda
python-intervaltree       3.0.2                      py_0    bioconda
python-msgpack            0.5.6            py27h470a237_0    bioconda
python-sortedcontainers   2.1.0                      py_0    bioconda
pytz                      2018.9                   py27_0  
readline                  7.0                  h7b6447c_5  
requests                  2.21.0                   py27_0  
samtools                  1.9                  h91753b0_5    bioconda
setuptools                40.8.0                   py27_0  
six                       1.12.0                   py27_0  
sqlite                    3.26.0               h7b6447c_0  
tk                        8.6.8                hbc83047_0  
traceback2                1.4.0                    py27_0  
unittest2                 1.1.0                    py27_0  
urllib3                   1.24.1                   py27_0  
wheel                     0.33.1                   py27_0  
xz                        5.2.4                h14c3975_4  
zlib                      1.2.11               h7b6447c_3  
@pb-cdunn
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This was originally PacificBiosciences/FALCON#685

I wrote:

You have to look into 0-rawreads/build. But please also post conda list. Someone else posted an odd bug, and I suspect that people are getting an inconsistent version of DAZZ_DB, which we provide via pb-dazzler.

You've got to look into 0-rawreads/build for stderr. You may have to debug this yourself by cd 0-rawreads/build and running parts of user_script.sh yourself. It will be difficult for us to debug this remotely, but with any luck, the answer will be obvious to you when to see stderr or run the commands yourself.

@NicMAlexandre
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NicMAlexandre commented Mar 13, 2019

stderr.txt

@pb-cdunn
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Here is the actual error, from near the bottom of your stderr file. (I copied your post and created a .txt file in WordPad, then copied that into GitHub for you.)

+ DBdust true raw_reads
Usage: DBdust [-b] [-w<int(64)>] [-t<double(2.)>] [-m<int(10)>] <path:db|dam>

      -w: DUST algorithm window size.
      -t: DUST algorithm threshold.
      -m: Record only low-complexity intervals >= this size.
      -b: Take into account base composition bias.

The current problem is that you are providing true to DBdust_option, rather than actual options. It is not a boolean setting.

@NicMAlexandre
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NicMAlexandre commented Mar 14, 2019 via email

@NicMAlexandre
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NicMAlexandre commented Mar 15, 2019 via email

@xiaoyezao
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xiaoyezao commented Mar 16, 2019

PacificBiosciences/FALCON#685

This was originally PacificBiosciences/FALCON#685

I wrote:

You have to look into 0-rawreads/build. But please also post conda list. Someone else posted an odd bug, and I suspect that people are getting an inconsistent version of DAZZ_DB, which we provide via pb-dazzler.

You've got to look into 0-rawreads/build for stderr. You may have to debug this yourself by cd 0-rawreads/build and running parts of user_script.sh yourself. It will be difficult for us to debug this remotely, but with any luck, the answer will be obvious to you when to see stderr or run the commands yourself.

Hi,
I got a similar issue to run pb-assembly in cluster.

I checked the stderr file in '0-rawreads/build', and found the following error message:

pypeflow 2.1.1
pypeflow 2.1.1
    raise Exception(msg)
Exception: Call 'bash -vex build_db.sh' returned 256.
2019-03-16 15:04:37,109 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
2019-03-16 15:04:37,109 - root - WARNING - CD: '/BIGDATA1/wbgcas_tfeng_1/project/pb_assembly_test/result/0-rawreads/build' -> '/BIGDATA1/wbgcas_tfeng_1/project/pb_assembly_test/result/0-rawreads/build'
2019-03-16 15:04:37,109 - root - WARNING - CD: '/BIGDATA1/wbgcas_tfeng_1/project/pb_assembly_test/result/0-rawreads/build' -> '/BIGDATA1/wbgcas_tfeng_1/project/pb_assembly_test/result/0-rawreads/build'
2019-03-16 15:04:37,109 - root - CRITICAL - Error in /BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/BIGDATA1/wbgcas_tfeng_1/project/pb_assembly_test/result/0-rawreads/build/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
Traceback (most recent call last):
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "__main__", fname, loader, pkg_name)
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in <module>
    main()
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py", line 259, in main
    run(**vars(parsed_args))
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py", line 253, in run
    run_cfg_in_tmpdir(cfg, tmpdir, '.')
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py", line 228, in run_cfg_in_tmpdir
    run_bash(bash_template, myinputs, myoutputs, parameters)
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/do_task.py", line 187, in run_bash
    util.system(cmd)
  File "/BIGDATA1/app/anaconda2/5.0.1-gcc-4.8.5/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)

How can I fix this ? Thanks

THIS IS THE DFG FILE:

Input

[General]
input_fofn=/BIGDATA1/wbgcas_tfeng_1/projcet/pb_assembly_test/input.fofn
input_type=raw
pa_DBdust_option=
pa_fasta_filter_option=pass
target=assembly
skip_checks=False
LA4Falcon_preload=false

#### Data Partitioning
pa_DBsplit_option=-x500 -s200
ovlp_DBsplit_option=-x500 -s200

#### Repeat Masking
pa_HPCTANmask_option=
pa_REPmask_code=0,300;0,300;0,300

####Pre-assembly
genome_size=0
seed_coverage=20
length_cutoff=1000    
pa_HPCdaligner_option=-v -B128 -M24
pa_daligner_option=-e.8 -l2000 -k18 -h480  -w8 -s100
falcon_sense_option=--output-multi --min-idt 0.70 --min-cov 2 --max-n-read 1800
falcon_sense_greedy=False

####Pread overlapping
ovlp_daligner_option=-e.9 -l2500 -k24 -h1024 -w6 -s100
ovlp_HPCdaligner_option=-v -B128 -M24

####Final Assembly
overlap_filtering_setting=--max-diff 100 --max-cov 100 --min-cov 2
fc_ovlp_to_graph_option=
length_cutoff_pr=1000

[job.defaults]
job_type=slurm
pwatcher_type=blocking
JOB_QUEUE = free
JOB_NAME = 200kb_test
MB = 32768
NPROC = 6
njobs = 32
submit = srun --wait=0 -p ${JOB_QUEUE} -J ${JOB_NAME} -o ${JOB_STDOUT} -e ${JOB_STDERR} --mem-per-cpu=${MB}M --cpus-per-task=${NPROC} ${JOB_SCRIPT}

[job.step.da]
NPROC=4
MB=32768
njobs=240
[job.step.la]
NPROC=4
MB=32768
njobs=240
[job.step.cns]
NPROC=8
MB=65536
njobs=240
[job.step.pda]
NPROC=4
MB=32768
njobs=240
[job.step.pla]
NPROC=4
MB=32768
njobs=240
[job.step.asm]
NPROC=24
MB=64608
njobs=1

@pb-cdunn
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I copied your message into WordPad and found this:

cat: write error: Broken pipe

There are a variety of ways that can happen, e.g. you could have run out of disk-space, or something could have killed one of the processes. You'll need to debug this at your end. Go into the failing task directory (0-rawreads/build/ I think), and run bash -vex user_script.sh. From there, you might be able to run specific bash commands and figure out what's going on.

@pb-cdunn pb-cdunn reopened this Mar 16, 2019
@NicMAlexandre
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NicMAlexandre commented Mar 18, 2019 via email

@pb-cdunn
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pb-cdunn commented Mar 18, 2019

Yep, you ran out of memory. And DBdust is single-threaded/non-parallel. (So using more machines would not help.) You can try different options to DBdust. (You can try to ask thegenemyers questions about that.) Or you can submit to a machine with more RAM. This is definitely not a Falcon error.

@NicMAlexandre
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With the following Config file:

Input for FALCON 3/18/19

[General]
input_fofn=fasta.fofn
input_type=raw
pa_DBdust_option=
pa_fasta_filter_option=streamed-median
target=assembly
skip_checks=False
LA4Falcon_preload=false
genome_size = 1100000000

Data Partitioning

pa_DBsplit_option=-x500 -s50
ovlp_DBsplit_option=-x500 -s50

Repeat Masking

pa_HPCTANmask_option=
pa_REPmask_code=0,300;0,300;0,300

####Pre-assembly
genome_size=1100000000
seed_coverage=30
length_cutoff=1000
pa_HPCdaligner_option=-v -B128 -M24
pa_daligner_option=-e.8 -l2000 -k18 -h480 -w8 -s100
falcon_sense_option=--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400
falcon_sense_greedy=False

####Pread overlapping
ovlp_daligner_option=-e.9 -l2500 -k24 -h1024 -w6 -s100
ovlp_HPCdaligner_option=-v -B128 -M24

####Final Assembly
overlap_filtering_setting=--max-diff 100 --max-cov 100 --min-cov 2
fc_ovlp_to_graph_option=
length_cutoff_pr=1000

[job.defaults]
pwatcher_type = blocking
submit = srun --wait 2
-p ${JOB_QUEUE}
-J ${JOB_NAME}
-o ${JOB_STDOUT}
-e ${JOB_STDERR}
--mem-per-cpu=${MB}M
--cpus-per-task=${NPROC}
${JOB_SCRIPT}
njobs = 32

And the following script:

#!/bin/bash

#SBATCH -p LM
#SBATCH -t 336:00:00
#SBATCH -N 1
#SBATCH --mem=3000GB
#SBATCH --cpus-per-task=4

source activate myenv

run it!

fc_run fc_run.cfg

I have the following new stderr output: Any help is appreciated.

executable=${PYPEFLOW_JOB_START_SCRIPT}

  • executable=/pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
  • timeout=60

Wait up to timeout seconds for the executable to become "executable",

then exec.

#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done

  • [[ ! -x /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash ]]
    executable=${PYPEFLOW_JOB_START_SCRIPT}
  • executable=/pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
  • timeout=60

Wait up to timeout seconds for the executable to become "executable",

then exec.

#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done

  • [[ ! -x /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash ]]
    executable=${PYPEFLOW_JOB_START_SCRIPT}
  • executable=/pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
  • timeout=60

Wait up to timeout seconds for the executable to become "executable",

then exec.

#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done

  • [[ ! -x /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash ]]

/bin/bash ${executable}

  • /bin/bash /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash

/bin/bash ${executable}

  • /bin/bash /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash

/bin/bash ${executable}

  • /bin/bash /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    executable=${PYPEFLOW_JOB_START_SCRIPT}
  • executable=/pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
  • timeout=60

Wait up to timeout seconds for the executable to become "executable",

then exec.

#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done

  • [[ ! -x /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash ]]

/bin/bash ${executable}

  • /bin/bash /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    executable=${PYPEFLOW_JOB_START_SCRIPT}
  • executable=/pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
    timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
  • timeout=60

Wait up to timeout seconds for the executable to become "executable",

then exec.

#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done

  • [[ ! -x /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash ]]

/bin/bash ${executable}

  • /bin/bash /pylon5/mc5fqip/orca21/0-rawreads/build/run-P2c7b8ae23d7f98.bash
  • '[' '!' -d /pylon5/mc5fqip/orca21/0-rawreads/build ']'
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • eval '/bin/bash run.sh'
    ++ /bin/bash run.sh
  • '[' '!' -d /pylon5/mc5fqip/orca21/0-rawreads/build ']'
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • eval '/bin/bash run.sh'
    ++ /bin/bash run.sh
  • '[' '!' -d /pylon5/mc5fqip/orca21/0-rawreads/build ']'
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • eval '/bin/bash run.sh'
    ++ /bin/bash run.sh
  • '[' '!' -d /pylon5/mc5fqip/orca21/0-rawreads/build ']'
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • eval '/bin/bash run.sh'
    ++ /bin/bash run.sh
  • '[' '!' -d /pylon5/mc5fqip/orca21/0-rawreads/build ']'
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • eval '/bin/bash run.sh'
    ++ /bin/bash run.sh
    export PATH=$PATH:/bin
  • export PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
  • PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
    cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
    /bin/bash task.sh
  • /bin/bash task.sh
    export PATH=$PATH:/bin
  • export PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
  • PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
    cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
    /bin/bash task.sh
  • /bin/bash task.sh
    export PATH=$PATH:/bin
  • export PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
  • PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
    cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
    /bin/bash task.sh
  • /bin/bash task.sh
    export PATH=$PATH:/bin
  • export PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
  • PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
    cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
    /bin/bash task.sh
  • /bin/bash task.sh
    export PATH=$PATH:/bin
  • export PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
  • PATH=/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/bin:/pylon5/mc5fqip/orca21/anaconda3/condabin:/home/orca21/.linuxbrew/Homebrew/sbin:/home/orca21/.linuxbrew/Homebrew/bin:/bin:/sbin:/pylon5/mc5fqip/orca21/anaconda3/bin:/pylon5/mc5fqip/orca21/bin:/usr/lib64/qt-3.3/bin:/opt/packages/xdusage/2.1-1/bin:/opt/intel/advisor_2018.4.0.574144/bin64:/opt/intel/vtune_amplifier_2018.4.0.573462/bin64:/opt/intel/inspector_2018.4.0.574143/bin64:/opt/intel/itac/2018.4.025/intel64/bin:/opt/intel/clck/2018.3/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/bin/intel64:/opt/intel/compilers_and_libraries_2018.5.274/linux/mpi/intel64/bin:/opt/packages/slurm/default/bin:/opt/packages/allocations:/opt/packages/interact/bin:/usr/lib64/ccache:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/opt/puppetlabs/puppet/bin:/opt/intel/parallel_studio_xe_2018.4.057/bin:/bin:/sbin:/opt/puppetlabs/bin:/home/orca21/.linuxbrew/Homebrew/bin:/home/orca21/bin:/home/orca21/.local/bin:/home/orca21/bin:/bin
    cd /pylon5/mc5fqip/orca21/0-rawreads/build
  • cd /pylon5/mc5fqip/orca21/0-rawreads/build
    /bin/bash task.sh
  • /bin/bash task.sh
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    2019-03-14 17:48:11,255 - root - DEBUG - Running "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py /pylon5/mc5fqip/orca21/0-rawreads/build/task.json"
    2019-03-14 17:48:11,255 - root - DEBUG - Running "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py /pylon5/mc5fqip/orca21/0-rawreads/build/task.json"
    2019-03-14 17:48:11,255 - root - DEBUG - Running "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py /pylon5/mc5fqip/orca21/0-rawreads/build/task.json"
    2019-03-14 17:48:11,255 - root - DEBUG - Running "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py /pylon5/mc5fqip/orca21/0-rawreads/build/task.json"
    2019-03-14 17:48:11,255 - root - DEBUG - Running "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py /pylon5/mc5fqip/orca21/0-rawreads/build/task.json"
    2019-03-14 17:48:11,259 - root - DEBUG - Checking existence of '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json' with timeout=30
    2019-03-14 17:48:11,260 - root - DEBUG - Loading JSON from '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json'
    2019-03-14 17:48:11,259 - root - DEBUG - Checking existence of '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json' with timeout=30
    2019-03-14 17:48:11,260 - root - DEBUG - Loading JSON from '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json'
    2019-03-14 17:48:11,259 - root - DEBUG - Checking existence of '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json' with timeout=30
    2019-03-14 17:48:11,260 - root - DEBUG - Loading JSON from '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json'
    2019-03-14 17:48:11,259 - root - DEBUG - Checking existence of '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json' with timeout=30
    2019-03-14 17:48:11,260 - root - DEBUG - Loading JSON from '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json'
    2019-03-14 17:48:11,259 - root - DEBUG - Checking existence of '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json' with timeout=30
    2019-03-14 17:48:11,260 - root - DEBUG - Loading JSON from '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json'
    2019-03-14 17:48:11,261 - root - DEBUG - {u'bash_template_fn': u'template.sh',
    u'inputs': {u'config': u'../../General_config.json',
    u'input_fofn': u'../../fasta.fofn'},
    u'outputs': {u'db': u'raw_reads.db', u'length_cutoff': u'length_cutoff'},
    u'parameters': {u'pypeflow_mb': 4000,
    u'pypeflow_nproc': 1,
    u'topdir': u'../..'}}
    2019-03-14 17:48:11,261 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,262 - root - DEBUG - Checking existence of u'../../fasta.fofn' with timeout=30
    2019-03-14 17:48:11,261 - root - DEBUG - {u'bash_template_fn': u'template.sh',
    u'inputs': {u'config': u'../../General_config.json',
    u'input_fofn': u'../../fasta.fofn'},
    u'outputs': {u'db': u'raw_reads.db', u'length_cutoff': u'length_cutoff'},
    u'parameters': {u'pypeflow_mb': 4000,
    u'pypeflow_nproc': 1,
    u'topdir': u'../..'}}
    2019-03-14 17:48:11,261 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,262 - root - DEBUG - Checking existence of u'../../fasta.fofn' with timeout=30
    2019-03-14 17:48:11,262 - root - DEBUG - {u'bash_template_fn': u'template.sh',
    u'inputs': {u'config': u'../../General_config.json',
    u'input_fofn': u'../../fasta.fofn'},
    u'outputs': {u'db': u'raw_reads.db', u'length_cutoff': u'length_cutoff'},
    u'parameters': {u'pypeflow_mb': 4000,
    u'pypeflow_nproc': 1,
    u'topdir': u'../..'}}
    2019-03-14 17:48:11,262 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,262 - root - DEBUG - {u'bash_template_fn': u'template.sh',
    u'inputs': {u'config': u'../../General_config.json',
    u'input_fofn': u'../../fasta.fofn'},
    u'outputs': {u'db': u'raw_reads.db', u'length_cutoff': u'length_cutoff'},
    u'parameters': {u'pypeflow_mb': 4000,
    u'pypeflow_nproc': 1,
    u'topdir': u'../..'}}
    2019-03-14 17:48:11,262 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,262 - root - DEBUG - Checking existence of u'../../fasta.fofn' with timeout=30
    2019-03-14 17:48:11,262 - root - DEBUG - Checking existence of u'../../fasta.fofn' with timeout=30
    2019-03-14 17:48:11,263 - root - DEBUG - {u'bash_template_fn': u'template.sh',
    u'inputs': {u'config': u'../../General_config.json',
    u'input_fofn': u'../../fasta.fofn'},
    u'outputs': {u'db': u'raw_reads.db', u'length_cutoff': u'length_cutoff'},
    u'parameters': {u'pypeflow_mb': 4000,
    u'pypeflow_nproc': 1,
    u'topdir': u'../..'}}
    2019-03-14 17:48:11,263 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,263 - root - DEBUG - Checking existence of u'../../fasta.fofn' with timeout=30
    2019-03-14 17:48:11,263 - root - DEBUG - Checking existence of u'../../General_config.json' with timeout=30
    2019-03-14 17:48:11,263 - root - DEBUG - Checking existence of u'../../General_config.json' with timeout=30
    2019-03-14 17:48:11,263 - root - DEBUG - Checking existence of u'../../General_config.json' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'../../General_config.json' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'../../General_config.json' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
    2019-03-14 17:48:11,264 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
    2019-03-14 17:48:11,265 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,265 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,266 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,266 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,266 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' <- '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:48:11,599 - root - INFO - $('/bin/bash user_script.sh')
    2019-03-14 17:48:11,599 - root - INFO - $('/bin/bash user_script.sh')
    2019-03-14 17:48:11,599 - root - INFO - $('/bin/bash user_script.sh')
    2019-03-14 17:48:11,599 - root - INFO - $('/bin/bash user_script.sh')
    2019-03-14 17:48:11,600 - root - INFO - $('/bin/bash user_script.sh')
    hostname
  • hostname
    hostname
  • hostname
    hostname
  • hostname
    hostname
  • hostname
    pwd
  • pwd
    date
  • date
    pwd
  • pwd
    date
  • date
    hostname
  • hostname
    pwd
  • pwd
    date
  • date
    pwd
  • pwd
    date
  • date
    pwd
  • pwd
    date
  • date

Substitution will be similar to snakemake "shell".

python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

  • python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

Substitution will be similar to snakemake "shell".

python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

  • python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

Substitution will be similar to snakemake "shell".

python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

  • python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

Substitution will be similar to snakemake "shell".

python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

  • python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

Substitution will be similar to snakemake "shell".

python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff

  • python -m falcon_kit.mains.dazzler --config-fn=../../General_config.json --db-fn=raw_reads.db build --input-fofn-fn=../../fasta.fofn --length-cutoff-fn=length_cutoff
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    [INFO]Log-level: INFO
    [INFO]Log-level: INFO
    [INFO]Log-level: INFO
    [INFO]Log-level: INFO
    [INFO]Log-level: INFO
    [INFO]config('../../General_config.json'):
    {u'falcon_sense_greedy': False, u'pa_fasta_filter_option': u'streamed-median', u'seed_coverage': u'30', u'pa_subsample_strategy': u'random', u'overlap_filtering_setting': u'--max-diff 100 --max-cov 100 --min-cov 2', u'length_cutoff_pr': u'1000', u'input_fofn': u'fasta.fofn', u'pa_HPCdaligner_option': u'-v -B128 -M24', u'pa_subsample_random_seed': 12345, u'ver': u'100', u'bestn': 12, u'pa_HPCTANmask_option': u'', u'input_type': u'raw', u'pa_REPmask_code': u'0,300;0,300;0,300', u'pa_DBsplit_option': u'-a -x500 -s200', u'fc_ovlp_to_graph_option': u' --min-len 1000', u'genome_size': u'1100000000', u'avoid_text_file_busy': True, u'pa_dazcon_option': u'-j 4 -x -l 500', u'ovlp_DBdust_option': u'', u'skip_checks': False, u'ovlp_daligner_option': u'-e.96 -s1000 -h60', u'target': u'assembly', u'falcon_sense_skip_contained': False, u'pa_daligner_option': u'-e0.8 -l2000 -k18 -h480 -w8 -s100', u'length_cutoff': u'-1', u'pa_DBdust_option': u'', u'ovlp_HPCdaligner_option': u'-v -M24 -l500', u'LA4Falcon_preload': False, u'pa_subsample_coverage': 0, u'dazcon': False, u'falcon_sense_option': u'--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400', u'ovlp_DBsplit_option': u'-x500 -s200'}
    [INFO]our subset of config:
    {'daligner_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 -v -B128 -M24', 'genome_size': 1100000000, 'TANmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_random_seed': 12345, 'DBdust_opt': u'', 'seed_coverage': 30.0, 'user_length_cutoff': -1, 'subsample_strategy': u'random', 'fasta_filter_option': u'streamed-median', 'REPmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_coverage': 0, 'DBsplit_opt': u'-a -x500 -s200'}
    [INFO]Building rdb from '../../fasta.fofn', to write 'raw_reads.db'
    [INFO]config('../../General_config.json'):
    {u'falcon_sense_greedy': False, u'pa_fasta_filter_option': u'streamed-median', u'seed_coverage': u'30', u'pa_subsample_strategy': u'random', u'overlap_filtering_setting': u'--max-diff 100 --max-cov 100 --min-cov 2', u'length_cutoff_pr': u'1000', u'input_fofn': u'fasta.fofn', u'pa_HPCdaligner_option': u'-v -B128 -M24', u'pa_subsample_random_seed': 12345, u'ver': u'100', u'bestn': 12, u'pa_HPCTANmask_option': u'', u'input_type': u'raw', u'pa_REPmask_code': u'0,300;0,300;0,300', u'pa_DBsplit_option': u'-a -x500 -s200', u'fc_ovlp_to_graph_option': u' --min-len 1000', u'genome_size': u'1100000000', u'avoid_text_file_busy': True, u'pa_dazcon_option': u'-j 4 -x -l 500', u'ovlp_DBdust_option': u'', u'skip_checks': False, u'ovlp_daligner_option': u'-e.96 -s1000 -h60', u'target': u'assembly', u'falcon_sense_skip_contained': False, u'pa_daligner_option': u'-e0.8 -l2000 -k18 -h480 -w8 -s100', u'length_cutoff': u'-1', u'pa_DBdust_option': u'', u'ovlp_HPCdaligner_option': u'-v -M24 -l500', u'LA4Falcon_preload': False, u'pa_subsample_coverage': 0, u'dazcon': False, u'falcon_sense_option': u'--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400', u'ovlp_DBsplit_option': u'-x500 -s200'}
    [INFO]our subset of config:
    {'daligner_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 -v -B128 -M24', 'genome_size': 1100000000, 'TANmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_random_seed': 12345, 'DBdust_opt': u'', 'seed_coverage': 30.0, 'user_length_cutoff': -1, 'subsample_strategy': u'random', 'fasta_filter_option': u'streamed-median', 'REPmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_coverage': 0, 'DBsplit_opt': u'-a -x500 -s200'}
    [INFO]Building rdb from '../../fasta.fofn', to write 'raw_reads.db'
    [INFO]config('../../General_config.json'):
    {u'falcon_sense_greedy': False, u'pa_fasta_filter_option': u'streamed-median', u'seed_coverage': u'30', u'pa_subsample_strategy': u'random', u'overlap_filtering_setting': u'--max-diff 100 --max-cov 100 --min-cov 2', u'length_cutoff_pr': u'1000', u'input_fofn': u'fasta.fofn', u'pa_HPCdaligner_option': u'-v -B128 -M24', u'pa_subsample_random_seed': 12345, u'ver': u'100', u'bestn': 12, u'pa_HPCTANmask_option': u'', u'input_type': u'raw', u'pa_REPmask_code': u'0,300;0,300;0,300', u'pa_DBsplit_option': u'-a -x500 -s200', u'fc_ovlp_to_graph_option': u' --min-len 1000', u'genome_size': u'1100000000', u'avoid_text_file_busy': True, u'pa_dazcon_option': u'-j 4 -x -l 500', u'ovlp_DBdust_option': u'', u'skip_checks': False, u'ovlp_daligner_option': u'-e.96 -s1000 -h60', u'target': u'assembly', u'falcon_sense_skip_contained': False, u'pa_daligner_option': u'-e0.8 -l2000 -k18 -h480 -w8 -s100', u'length_cutoff': u'-1', u'pa_DBdust_option': u'', u'ovlp_HPCdaligner_option': u'-v -M24 -l500', u'LA4Falcon_preload': False, u'pa_subsample_coverage': 0, u'dazcon': False, u'falcon_sense_option': u'--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400', u'ovlp_DBsplit_option': u'-x500 -s200'}
    [INFO]our subset of config:
    {'daligner_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 -v -B128 -M24', 'genome_size': 1100000000, 'TANmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_random_seed': 12345, 'DBdust_opt': u'', 'seed_coverage': 30.0, 'user_length_cutoff': -1, 'subsample_strategy': u'random', 'fasta_filter_option': u'streamed-median', 'REPmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_coverage': 0, 'DBsplit_opt': u'-a -x500 -s200'}
    [INFO]Building rdb from '../../fasta.fofn', to write 'raw_reads.db'
    [INFO]config('../../General_config.json'):
    {u'falcon_sense_greedy': False, u'pa_fasta_filter_option': u'streamed-median', u'seed_coverage': u'30', u'pa_subsample_strategy': u'random', u'overlap_filtering_setting': u'--max-diff 100 --max-cov 100 --min-cov 2', u'length_cutoff_pr': u'1000', u'input_fofn': u'fasta.fofn', u'pa_HPCdaligner_option': u'-v -B128 -M24', u'pa_subsample_random_seed': 12345, u'ver': u'100', u'bestn': 12, u'pa_HPCTANmask_option': u'', u'input_type': u'raw', u'pa_REPmask_code': u'0,300;0,300;0,300', u'pa_DBsplit_option': u'-a -x500 -s200', u'fc_ovlp_to_graph_option': u' --min-len 1000', u'genome_size': u'1100000000', u'avoid_text_file_busy': True, u'pa_dazcon_option': u'-j 4 -x -l 500', u'ovlp_DBdust_option': u'', u'skip_checks': False, u'ovlp_daligner_option': u'-e.96 -s1000 -h60', u'target': u'assembly', u'falcon_sense_skip_contained': False, u'pa_daligner_option': u'-e0.8 -l2000 -k18 -h480 -w8 -s100', u'length_cutoff': u'-1', u'pa_DBdust_option': u'', u'ovlp_HPCdaligner_option': u'-v -M24 -l500', u'LA4Falcon_preload': False, u'pa_subsample_coverage': 0, u'dazcon': False, u'falcon_sense_option': u'--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400', u'ovlp_DBsplit_option': u'-x500 -s200'}
    [INFO]our subset of config:
    {'daligner_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 -v -B128 -M24', 'genome_size': 1100000000, 'TANmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_random_seed': 12345, 'DBdust_opt': u'', 'seed_coverage': 30.0, 'user_length_cutoff': -1, 'subsample_strategy': u'random', 'fasta_filter_option': u'streamed-median', 'REPmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_coverage': 0, 'DBsplit_opt': u'-a -x500 -s200'}
    [INFO]Building rdb from '../../fasta.fofn', to write 'raw_reads.db'
    [INFO]config('../../General_config.json'):
    {u'falcon_sense_greedy': False, u'pa_fasta_filter_option': u'streamed-median', u'seed_coverage': u'30', u'pa_subsample_strategy': u'random', u'overlap_filtering_setting': u'--max-diff 100 --max-cov 100 --min-cov 2', u'length_cutoff_pr': u'1000', u'input_fofn': u'fasta.fofn', u'pa_HPCdaligner_option': u'-v -B128 -M24', u'pa_subsample_random_seed': 12345, u'ver': u'100', u'bestn': 12, u'pa_HPCTANmask_option': u'', u'input_type': u'raw', u'pa_REPmask_code': u'0,300;0,300;0,300', u'pa_DBsplit_option': u'-a -x500 -s200', u'fc_ovlp_to_graph_option': u' --min-len 1000', u'genome_size': u'1100000000', u'avoid_text_file_busy': True, u'pa_dazcon_option': u'-j 4 -x -l 500', u'ovlp_DBdust_option': u'', u'skip_checks': False, u'ovlp_daligner_option': u'-e.96 -s1000 -h60', u'target': u'assembly', u'falcon_sense_skip_contained': False, u'pa_daligner_option': u'-e0.8 -l2000 -k18 -h480 -w8 -s100', u'length_cutoff': u'-1', u'pa_DBdust_option': u'', u'ovlp_HPCdaligner_option': u'-v -M24 -l500', u'LA4Falcon_preload': False, u'pa_subsample_coverage': 0, u'dazcon': False, u'falcon_sense_option': u'--output-multi --min-idt 0.70 --min-cov 3 --max-n-read 400', u'ovlp_DBsplit_option': u'-x500 -s200'}
    [INFO]our subset of config:
    {'daligner_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 -v -B128 -M24', 'genome_size': 1100000000, 'TANmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_random_seed': 12345, 'DBdust_opt': u'', 'seed_coverage': 30.0, 'user_length_cutoff': -1, 'subsample_strategy': u'random', 'fasta_filter_option': u'streamed-median', 'REPmask_opt': u'-e0.8 -l2000 -k18 -h480 -w8 -s100 ', 'subsample_coverage': 0, 'DBsplit_opt': u'-a -x500 -s200'}
    [INFO]Building rdb from '../../fasta.fofn', to write 'raw_reads.db'
    [INFO]$('bash -vex build_db.sh')
    [INFO]$('bash -vex build_db.sh')
    [INFO]$('bash -vex build_db.sh')
    [INFO]$('bash -vex build_db.sh')
    [INFO]$('bash -vex build_db.sh')
    module () { eval /usr/bin/modulecmd bash $*
    }
    module () { eval /usr/bin/modulecmd bash $*
    }
    #!/bin/bash
    set -vex
  • set -vex
    echo "PBFALCON_ERRFILE=$PBFALCON_ERRFILE"
  • echo PBFALCON_ERRFILE=
    set -o pipefail
  • set -o pipefail
    rm -f raw_reads.db raw_reads.dam .raw_reads.* # in case of re-run
    #!/bin/bash
    set -vex
  • set -vex
    echo "PBFALCON_ERRFILE=$PBFALCON_ERRFILE"
  • echo PBFALCON_ERRFILE=
    set -o pipefail
  • set -o pipefail
    rm -f raw_reads.db raw_reads.dam .raw_reads.* # in case of re-run
    module () { eval /usr/bin/modulecmd bash $*
    }
    #!/bin/bash
    set -vex
  • set -vex
    echo "PBFALCON_ERRFILE=$PBFALCON_ERRFILE"
  • echo PBFALCON_ERRFILE=
    set -o pipefail
  • set -o pipefail
    rm -f raw_reads.db raw_reads.dam .raw_reads.* # in case of re-run
    module () { eval /usr/bin/modulecmd bash $*
    }
    #!/bin/bash
    set -vex
  • set -vex
    echo "PBFALCON_ERRFILE=$PBFALCON_ERRFILE"
  • echo PBFALCON_ERRFILE=
    set -o pipefail
  • set -o pipefail
    module () { eval /usr/bin/modulecmd bash $*
    rm -f raw_reads.db raw_reads.dam .raw_reads.* # in case of re-run
    }
    #!/bin/bash
    set -vex
  • set -vex
    echo "PBFALCON_ERRFILE=$PBFALCON_ERRFILE"
  • echo PBFALCON_ERRFILE=
    set -o pipefail
  • set -o pipefail
    rm -f raw_reads.db raw_reads.dam .raw_reads.* # in case of re-run
  • rm -f raw_reads.db raw_reads.dam .raw_reads.bps .raw_reads.idx
  • rm -f raw_reads.db raw_reads.dam .raw_reads.bps .raw_reads.idx
  • rm -f raw_reads.db raw_reads.dam .raw_reads.bps .raw_reads.idx
  • rm -f raw_reads.db raw_reads.dam .raw_reads.bps .raw_reads.idx
  • rm -f raw_reads.db raw_reads.dam .raw_reads.bps .raw_reads.idx
    #fc_fasta2fasta < my.fasta.fofn >| fc.fofn
    zmw_whitelist_option=""
  • zmw_whitelist_option=
    use_subsampling=0
    #fc_fasta2fasta < my.fasta.fofn >| fc.fofn
    zmw_whitelist_option=""
  • use_subsampling=0
    if [[ $use_subsampling -eq 1 ]]; then
    python -m falcon_kit.mains.fasta_subsample --coverage "0" --random-seed "12345" --strategy "random" --genome-size "1100000000" "my.fasta.fofn" zmw
    zmw_whitelist_option="--zmw-whitelist-fn zmw.whitelist.json"
    fi
  • zmw_whitelist_option=
    use_subsampling=0
  • use_subsampling=0
    if [[ $use_subsampling -eq 1 ]]; then
    python -m falcon_kit.mains.fasta_subsample --coverage "0" --random-seed "12345" --strategy "random" --genome-size "1100000000" "my.fasta.fofn" zmw
  • [[ 0 -eq 1 ]]
    while read fn; do cat ${fn} | python -m falcon_kit.mains.fasta_filter ${zmw_whitelist_option} streamed-median - | fasta2DB -v raw_reads -i${fn##*/}; done < my.fasta.fofn
    #fc_fasta2fasta < my.fasta.fofn >| fc.fofn
    zmw_whitelist_option=""
    zmw_whitelist_option="--zmw-whitelist-fn zmw.whitelist.json"
    fi
  • [[ 0 -eq 1 ]]
  • zmw_whitelist_option=
    use_subsampling=0
  • use_subsampling=0
    while read fn; do cat ${fn} | python -m falcon_kit.mains.fasta_filter ${zmw_whitelist_option} streamed-median - | fasta2DB -v raw_reads -i${fn##*/}; done < my.fasta.fofn
    if [[ $use_subsampling -eq 1 ]]; then
    python -m falcon_kit.mains.fasta_subsample --coverage "0" --random-seed "12345" --strategy "random" --genome-size "1100000000" "my.fasta.fofn" zmw
    zmw_whitelist_option="--zmw-whitelist-fn zmw.whitelist.json"
    fi
  • [[ 0 -eq 1 ]]
    while read fn; do cat ${fn} | python -m falcon_kit.mains.fasta_filter ${zmw_whitelist_option} streamed-median - | fasta2DB -v raw_reads -i${fn##*/}; done < my.fasta.fofn
  • read fn
  • cat ../../m54050R1_180201_210150.subreads.fasta
    #fc_fasta2fasta < my.fasta.fofn >| fc.fofn
    zmw_whitelist_option=""
  • zmw_whitelist_option=
    use_subsampling=0
  • use_subsampling=0
    if [[ $use_subsampling -eq 1 ]]; then
    python -m falcon_kit.mains.fasta_subsample --coverage "0" --random-seed "12345" --strategy "random" --genome-size "1100000000" "my.fasta.fofn" zmw
    zmw_whitelist_option="--zmw-whitelist-fn zmw.whitelist.json"
    fi
  • [[ 0 -eq 1 ]]
    while read fn; do cat ${fn} | python -m falcon_kit.mains.fasta_filter ${zmw_whitelist_option} streamed-median - | fasta2DB -v raw_reads -i${fn##*/}; done < my.fasta.fofn
  • read fn
  • read fn
  • read fn
  • fasta2DB -v raw_reads -im54050R1_180201_210150.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180201_210150.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • cat ../../m54050R1_180201_210150.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180201_210150.subreads.fasta
    #fc_fasta2fasta < my.fasta.fofn >| fc.fofn
    zmw_whitelist_option=""
  • zmw_whitelist_option=
    use_subsampling=0
  • use_subsampling=0
    if [[ $use_subsampling -eq 1 ]]; then
    python -m falcon_kit.mains.fasta_subsample --coverage "0" --random-seed "12345" --strategy "random" --genome-size "1100000000" "my.fasta.fofn" zmw
    zmw_whitelist_option="--zmw-whitelist-fn zmw.whitelist.json"
    fi
  • [[ 0 -eq 1 ]]
    while read fn; do cat ${fn} | python -m falcon_kit.mains.fasta_filter ${zmw_whitelist_option} streamed-median - | fasta2DB -v raw_reads -i${fn##*/}; done < my.fasta.fofn
  • read fn
  • cat ../../m54050R1_180201_210150.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180201_210150.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180201_210150.subreads.fasta
  • cat ../../m54050R1_180201_210150.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • cat ../../m54050R1_180201_210150.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180201_210150.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180201_210150.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180201_210150.subreads.fasta
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 224
    INFO:root: Consistency and FAQs #2 count= 839
    INFO:root: Python3 compatibility #3 count= 2,102
    INFO:root: Add license #4 count= 4,170
    INFO:root: variantCaller Error #6 count= 8,348
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9 count= 14,686
    INFO:root: bax2bam: symbol lookup error: ../lib/libblasr.so.5.3.1: undefined symbol: _ZNK2H510H5Location15getObjnameByIdxEy #11 count= 28,274
    INFO:root: pb-assembly failing/stalling in cns-runs #113 count= 48,575
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 224
    INFO:root: Consistency and FAQs #2 count= 839
    INFO:root: Python3 compatibility #3 count= 2,102
    INFO:root: Add license #4 count= 4,170
    INFO:root: variantCaller Error #6 count= 8,348
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9 count= 14,686
    INFO:root: bax2bam: symbol lookup error: ../lib/libblasr.so.5.3.1: undefined symbol: _ZNK2H510H5Location15getObjnameByIdxEy #11 count= 28,274
    INFO:root: pb-assembly failing/stalling in cns-runs #113 count= 48,575
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 224
    INFO:root: Consistency and FAQs #2 count= 839
    INFO:root: Python3 compatibility #3 count= 2,102
    INFO:root: Add license #4 count= 4,170
    INFO:root: variantCaller Error #6 count= 8,348
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9 count= 14,686
    INFO:root: bax2bam: symbol lookup error: ../lib/libblasr.so.5.3.1: undefined symbol: _ZNK2H510H5Location15getObjnameByIdxEy #11 count= 28,274
    INFO:root: pb-assembly failing/stalling in cns-runs #113 count= 48,575
    Adding 'm54050R1_180201_210150.subreads.fasta.fasta' ...
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 224
    INFO:root: Consistency and FAQs #2 count= 839
    INFO:root: Python3 compatibility #3 count= 2,102
    INFO:root: Add license #4 count= 4,170
    INFO:root: variantCaller Error #6 count= 8,348
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9 count= 14,686
    INFO:root: bax2bam: symbol lookup error: ../lib/libblasr.so.5.3.1: undefined symbol: _ZNK2H510H5Location15getObjnameByIdxEy #11 count= 28,274
    INFO:root: pb-assembly failing/stalling in cns-runs #113 count= 48,575
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 224
    INFO:root: Consistency and FAQs #2 count= 839
    INFO:root: Python3 compatibility #3 count= 2,102
    INFO:root: Add license #4 count= 4,170
    INFO:root: variantCaller Error #6 count= 8,348
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9 count= 14,686
    INFO:root: bax2bam: symbol lookup error: ../lib/libblasr.so.5.3.1: undefined symbol: _ZNK2H510H5Location15getObjnameByIdxEy #11 count= 28,274
    INFO:root: pb-assembly failing/stalling in cns-runs #113 count= 48,575
    Adding 'm54050R1_180201_210150.subreads.fasta.fasta' ...
    Adding 'm54050R1_180201_210150.subreads.fasta.fasta' ...
    INFO:root: Only have fastq subreads to polish contigs using pbmm2 and gcpp #317 count= 89,000
    INFO:root: Only have fastq subreads to polish contigs using pbmm2 and gcpp #317 count= 89,000
    Adding 'm54050R1_180201_210150.subreads.fasta.fasta' ...
    Adding 'm54050R1_180201_210150.subreads.fasta.fasta' ...
    INFO:root: Only have fastq subreads to polish contigs using pbmm2 and gcpp #317 count= 89,000
    INFO:root: Only have fastq subreads to polish contigs using pbmm2 and gcpp #317 count= 89,000
    INFO:root: Only have fastq subreads to polish contigs using pbmm2 and gcpp #317 count= 89,000
    INFO:root: All reads marked as Without SMRTbell adapter in lima #492 count= 178,009
    INFO:root: All reads marked as Without SMRTbell adapter in lima #492 count= 178,009
    INFO:root: All reads marked as Without SMRTbell adapter in lima #492 count= 178,009
    INFO:root: All reads marked as Without SMRTbell adapter in lima #492 count= 178,009
    INFO:root: All reads marked as Without SMRTbell adapter in lima #492 count= 178,009
    INFO:root: Unknown subcommand "ipa2-construct-config" #507 count= 355,936
    INFO:root: Unknown subcommand "ipa2-construct-config" #507 count= 355,936
    INFO:root: Unknown subcommand "ipa2-construct-config" #507 count= 355,936
    INFO:root: Unknown subcommand "ipa2-construct-config" #507 count= 355,936
    INFO:root: Unknown subcommand "ipa2-construct-config" #507 count= 355,936
    INFO:root: Error in rule phasing_prepare #612 count= 692,452
    INFO:root: Error in rule phasing_prepare #612 count= 692,452
    INFO:root: Error in rule phasing_prepare #612 count= 692,452
    INFO:root: Error in rule phasing_prepare #612 count= 692,452
    INFO:root: Error in rule phasing_prepare #612 count= 692,452
    INFO:root: Generate HiFi reads #687 count= 1,370,718
    INFO:root: Generate HiFi reads #687 count= 1,370,718
    INFO:root: Generate HiFi reads #687 count= 1,370,718
    INFO:root: Generate HiFi reads #687 count= 1,370,718
    INFO:root: Generate HiFi reads #687 count= 1,370,718
    INFO:root: #816 count= 2,688,290
    INFO:root: #816 count= 2,688,290
    INFO:root: #816 count= 2,688,290
    INFO:root: #816 count= 2,688,290
    INFO:root: #816 count= 2,688,290
    INFO:root: Update issue templates #1,375 count= 5,277,506
    INFO:root: Update issue templates #1,375 count= 5,277,506
    INFO:root: Update issue templates #1,375 count= 5,277,506
    INFO:root: Update issue templates #1,375 count= 5,277,506
    INFO:root: Update issue templates #1,375 count= 5,277,506
    INFO:root: Consistency and FAQs #2,367 count= 10,451,057
    INFO:root: Consistency and FAQs #2,367 count= 10,451,057
    INFO:root: Consistency and FAQs #2,367 count= 10,451,057
    INFO:root: Consistency and FAQs #2,367 count= 10,451,057
    INFO:root: Consistency and FAQs #2,367 count= 10,451,057
    INFO:root: Add license #4,437 count= 20,814,122
    INFO:root: Add license #4,437 count= 20,814,122
    INFO:root: Add license #4,437 count= 20,814,122
    INFO:root: Add license #4,437 count= 20,814,122
    INFO:root: Add license #4,437 count= 20,814,122
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9,932 count= 41,507,957
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9,932 count= 41,507,957
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9,932 count= 41,507,957
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9,932 count= 41,507,957
    INFO:root: falcon_kit.mains.fasta_filter is crashing #9,932 count= 41,507,957
    INFO:root: bax2bam: libpbbam.so.0.18.0 is missing #17,666 count= 82,897,567
    INFO:root: bax2bam: libpbbam.so.0.18.0 is missing #17,666 count= 82,897,567
    INFO:root: bax2bam: libpbbam.so.0.18.0 is missing #17,666 count= 82,897,567
    INFO:root: bax2bam: libpbbam.so.0.18.0 is missing #17,666 count= 82,897,567
    INFO:root: bax2bam: libpbbam.so.0.18.0 is missing #17,666 count= 82,897,567
    INFO:root: download link for model.json needed for unanimity #32,172 count= 165,669,678
    INFO:root: download link for model.json needed for unanimity #32,172 count= 165,669,678
    INFO:root: download link for model.json needed for unanimity #32,172 count= 165,669,678
    INFO:root: download link for model.json needed for unanimity #32,172 count= 165,669,678
    INFO:root: download link for model.json needed for unanimity #32,172 count= 165,669,678
    INFO:root: pbmm2 M5 field in BAM header missing #58,663 count= 331,230,374
    INFO:root: pbmm2 M5 field in BAM header missing #58,663 count= 331,230,374
    INFO:root: pbmm2 M5 field in BAM header missing #58,663 count= 331,230,374
    INFO:root: pbmm2 M5 field in BAM header missing #58,663 count= 331,230,374
    INFO:root: pbmm2 M5 field in BAM header missing #58,663 count= 331,230,374
    INFO:root: fc_run ERROR: CRITICAL - Error in ... #111,773 count= 662,326,916
    INFO:root: fc_run ERROR: CRITICAL - Error in ... #111,773 count= 662,326,916
    INFO:root: fc_run ERROR: CRITICAL - Error in ... #111,773 count= 662,326,916
    INFO:root: fc_run ERROR: CRITICAL - Error in ... #111,773 count= 662,326,916
    INFO:root: fc_run ERROR: CRITICAL - Error in ... #111,773 count= 662,326,916
    INFO:root: pbmm2 align error #228,302 count= 1,324,520,840
    INFO:root: pbmm2 align error #228,302 count= 1,324,520,840
    INFO:root: pbmm2 align error #228,302 count= 1,324,520,840
    INFO:root: pbmm2 align error #228,302 count= 1,324,520,840
    INFO:root: pbmm2 align error #228,302 count= 1,324,520,840
    INFO:root: #456,327 count= 2,648,877,672
    INFO:root: #456,327 count= 2,648,877,672
    INFO:root: #456,327 count= 2,648,877,672
    INFO:root: #456,327 count= 2,648,877,672
    INFO:root: #456,327 count= 2,648,877,672
  • read fn
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • cat ../../m54050R1_180205_001444.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180205_001444.subreads.fasta
  • read fn
  • read fn
  • read fn
  • cat ../../m54050R1_180205_001444.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_001444.subreads.fasta
  • cat ../../m54050R1_180205_001444.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_001444.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180205_001444.subreads.fasta
  • cat ../../m54050R1_180205_001444.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • read fn
  • cat ../../m54050R1_180205_001444.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_001444.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_001444.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_001444.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_001444.subreads.fasta
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_001444.subreads.fasta
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 14,054
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 14,054
    INFO:root: Python3 compatibility #3 count= 44,205
    Adding 'm54050R1_180205_001444.subreads.fasta.fasta' ...
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 14,054
    INFO:root: Python3 compatibility #3 count= 44,205
    INFO:root: Python3 compatibility #3 count= 44,205
    Adding 'm54050R1_180205_001444.subreads.fasta.fasta' ...
    INFO:root: Issue with FASTA reference for arrow #5 count= 99,813
    Adding 'm54050R1_180205_001444.subreads.fasta.fasta' ...
    INFO:root: Issue with FASTA reference for arrow #5 count= 99,813
    INFO:root: variantCaller Error #6 count= 164,238
    INFO:root: Issue with FASTA reference for arrow #5 count= 99,813
    INFO:root: variantCaller Error #6 count= 164,238
    INFO:root:Counting bytes from "None"
    INFO:root: variantCaller Error #6 count= 164,238
    INFO:root: Update issue templates #1 count= 14,054
    INFO:root: Python3 compatibility #3 count= 44,205
    Adding 'm54050R1_180205_001444.subreads.fasta.fasta' ...
    INFO:root: Issue with FASTA reference for arrow #5 count= 99,813
    INFO:root: variantCaller Error #6 count= 164,238
    INFO:root: PB-Assembly #12 count= 285,279
    INFO:root: PB-Assembly #12 count= 285,279
    INFO:root: PB-Assembly #12 count= 285,279
    INFO:root: PB-Assembly #12 count= 285,279
    INFO:root: Need a mechanism to better control what filesystems are used for running certain steps. #36 count= 538,708
    INFO:root: Need a mechanism to better control what filesystems are used for running certain steps. #36 count= 538,708
    pypeflow 2.1.1
    INFO:root: Need a mechanism to better control what filesystems are used for running certain steps. #36 count= 538,708
    INFO:root: Need a mechanism to better control what filesystems are used for running certain steps. #36 count= 538,708
    INFO:root: pbmm2 primary alignments #128 count= 996,561
    INFO:root: pbmm2 primary alignments #128 count= 996,561
    INFO:root: pbmm2 primary alignments #128 count= 996,561
    INFO:root: pbmm2 primary alignments #128 count= 996,561
    INFO:root: Recommendation for tools to detect tandem repeats? #233 count= 1,916,542
    INFO:root: Recommendation for tools to detect tandem repeats? #233 count= 1,916,542
    INFO:root: Recommendation for tools to detect tandem repeats? #233 count= 1,916,542
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 14,054
    INFO:root: Python3 compatibility #3 count= 44,205
    Adding 'm54050R1_180205_001444.subreads.fasta.fasta' ...
    INFO:root: Issue with FASTA reference for arrow #5 count= 99,813
    INFO:root: Recommendation for tools to detect tandem repeats? #233 count= 1,916,542
    INFO:root: variantCaller Error #6 count= 164,238
    INFO:root: PB-Assembly #12 count= 285,279
    INFO:root: Need a mechanism to better control what filesystems are used for running certain steps. #36 count= 538,708
    INFO:root: pbmm2 primary alignments #128 count= 996,561
    INFO:root: Somatic variant calling with pbsv and ploidy consideration #359 count= 3,717,003
    INFO:root: Somatic variant calling with pbsv and ploidy consideration #359 count= 3,717,003
    INFO:root: Somatic variant calling with pbsv and ploidy consideration #359 count= 3,717,003
    INFO:root: Somatic variant calling with pbsv and ploidy consideration #359 count= 3,717,003
    INFO:root: Recommendation for tools to detect tandem repeats? #233 count= 1,916,542
    INFO:root: Somatic variant calling with pbsv and ploidy consideration #359 count= 3,717,003
    INFO:root: Update issue templates #1,096 count= 7,315,096
    INFO:root: Update issue templates #1,096 count= 7,315,096
    INFO:root: Update issue templates #1,096 count= 7,315,096
    INFO:root: Update issue templates #1,096 count= 7,315,096
    INFO:root: Update issue templates #1,096 count= 7,315,096
    INFO:root: Consistency and FAQs #2,198 count= 14,516,279
    INFO:root: Consistency and FAQs #2,198 count= 14,516,279
    INFO:root: Consistency and FAQs #2,198 count= 14,516,279
    INFO:root: Consistency and FAQs #2,198 count= 14,516,279
    INFO:root: Consistency and FAQs #2,198 count= 14,516,279
    INFO:root: Issue with FASTA reference for arrow #5,484 count= 28,909,411
    INFO:root: Issue with FASTA reference for arrow #5,484 count= 28,909,411
    INFO:root: Issue with FASTA reference for arrow #5,484 count= 28,909,411
    INFO:root: Issue with FASTA reference for arrow #5,484 count= 28,909,411
    INFO:root: Issue with FASTA reference for arrow #5,484 count= 28,909,411
    INFO:root: pb-assembly falcon does not run with slurm #10,469 count= 57,692,955
    INFO:root: pb-assembly falcon does not run with slurm #10,469 count= 57,692,955
    INFO:root: pb-assembly falcon does not run with slurm #10,469 count= 57,692,955
    INFO:root: pb-assembly falcon does not run with slurm #10,469 count= 57,692,955
    INFO:root: pb-assembly falcon does not run with slurm #10,469 count= 57,692,955
    INFO:root: [ERROR]Task Node(0-rawreads/build) failed with exit-code=1 #18,997 count= 115,259,072
    INFO:root: [ERROR]Task Node(0-rawreads/build) failed with exit-code=1 #18,997 count= 115,259,072
    INFO:root: [ERROR]Task Node(0-rawreads/build) failed with exit-code=1 #18,997 count= 115,259,072
    INFO:root: [ERROR]Task Node(0-rawreads/build) failed with exit-code=1 #18,997 count= 115,259,072
    INFO:root: [ERROR]Task Node(0-rawreads/build) failed with exit-code=1 #18,997 count= 115,259,072
    INFO:root: CONDA Solving environment: failed #37,811 count= 230,392,828
    INFO:root: CONDA Solving environment: failed #37,811 count= 230,392,828
    INFO:root: CONDA Solving environment: failed #37,811 count= 230,392,828
    INFO:root: CONDA Solving environment: failed #37,811 count= 230,392,828
    INFO:root: CONDA Solving environment: failed #37,811 count= 230,392,828
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71,452 count= 460,654,500
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71,452 count= 460,654,500
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71,452 count= 460,654,500
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71,452 count= 460,654,500
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71,452 count= 460,654,500
    INFO:root: pbmm2 truncated output.bam #143,311 count= 921,176,072
    INFO:root: pbmm2 truncated output.bam #143,311 count= 921,176,072
    INFO:root: pbmm2 truncated output.bam #143,311 count= 921,176,072
    INFO:root: pbmm2 truncated output.bam #143,311 count= 921,176,072
    INFO:root: pbmm2 truncated output.bam #143,311 count= 921,176,072
    INFO:root: pbmerge after pbmm2 #301,804 count= 1,842,225,458
    INFO:root: pbmerge after pbmm2 #301,804 count= 1,842,225,458
    INFO:root: pbmerge after pbmm2 #301,804 count= 1,842,225,458
    INFO:root: pbmerge after pbmm2 #301,804 count= 1,842,225,458
    INFO:root: pbmerge after pbmm2 #301,804 count= 1,842,225,458
    INFO:root: Identification of structural variants in simulated genome by pbsv #632,056 count= 3,684,320,381
    INFO:root: Identification of structural variants in simulated genome by pbsv #632,056 count= 3,684,320,381
    INFO:root: Identification of structural variants in simulated genome by pbsv #632,056 count= 3,684,320,381
    INFO:root: Identification of structural variants in simulated genome by pbsv #632,056 count= 3,684,320,381
    INFO:root: Identification of structural variants in simulated genome by pbsv #632,056 count= 3,684,320,381
  • read fn
  • read fn
  • cat ../../m54050R1_180205_102507.subreads.fasta
  • cat ../../m54050R1_180205_102507.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_102507.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_102507.subreads.fasta
  • read fn
  • cat ../../m54050R1_180205_102507.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • read fn
  • fasta2DB -v raw_reads -im54050R1_180205_102507.subreads.fasta
  • cat ../../m54050R1_180205_102507.subreads.fasta
  • fasta2DB -v raw_reads -im54050R1_180205_102507.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • read fn
  • cat ../../m54050R1_180205_102507.subreads.fasta
  • python -m falcon_kit.mains.fasta_filter streamed-median -
  • fasta2DB -v raw_reads -im54050R1_180205_102507.subreads.fasta
    fasta2DB: raw_reads.db is corrupted, read failed
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_102507.subreads.fasta
    fasta2DB: raw_reads.db is corrupted, read failed
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_102507.subreads.fasta
    fasta2DB: raw_reads.db is corrupted, read failed
    fasta2DB: [WARNING] Could not delete temporary file m54050R1_180205_102507.subreads.fasta
    fasta2DB: raw_reads.db is corrupted, read failed
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    falcon-kit 1.2.6
    pypeflow 2.1.1
    pypeflow 2.1.1
    pypeflow 2.1.1
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 201
    INFO:root:Counting bytes from "None"
    INFO:root: Consistency and FAQs #2 count= 422
    INFO:root: Add license #4 count= 884
    INFO:root: isoseq3 package not found at bioconda #8 count= 1,773
    INFO:root: blasr bam output problem #15 count= 3,383
    INFO:root: Update issue templates #1 count= 201
    INFO:root: Consistency and FAQs #2 count= 422
    INFO:root: Add license #4 count= 884
    INFO:root: isoseq3 package not found at bioconda #8 count= 1,773
    INFO:root: blasr bam output problem #15 count= 3,383
    INFO:root: Updating metadata for pbbam package #29 count= 6,612
    INFO:root: Falcon: parameters to optimize haplotype resolution #57 count= 13,179
    INFO:root: Iso-Seq 3 cluster fails with segfault in clustering #61 count= 37,191
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    pypeflow 2.1.1
    INFO:root: Updating metadata for pbbam package #29 count= 6,612
    INFO:root: Falcon: parameters to optimize haplotype resolution #57 count= 13,179
    INFO:root: Iso-Seq 3 cluster fails with segfault in clustering #61 count= 37,191
    Adding 'm54050R1_180205_102507.subreads.fasta.fasta' ...
    INFO:root: Missing pytz dependency for pb-assembly #63 count= 76,347
    INFO:root: Is HGAP4 output compatible with pb-assembly? #71 count= 134,764
    pypeflow 2.1.1
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 201
    INFO:root: Consistency and FAQs #2 count= 422
    INFO:root: Add license #4 count= 884
    INFO:root: isoseq3 package not found at bioconda #8 count= 1,773
    INFO:root: blasr bam output problem #15 count= 3,383
    INFO:root: Updating metadata for pbbam package #29 count= 6,612
    INFO:root: Falcon: parameters to optimize haplotype resolution #57 count= 13,179
    INFO:root: Iso-Seq 3 cluster fails with segfault in clustering #61 count= 37,191
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    INFO:root: FR: temp directory parameter for ccs #425 count= 243,370
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    "main", fname, loader, pkg_name)
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 357, in
    exec code in run_globals
    main(sys.argv) # pragma: no cover
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 354, in main
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 267, in cmd_run_streamed_median_filter
    run_streamed_median_filter(fp_in, sys.stdout, whitelist_set)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 147, in run_streamed_median_filter
    write_streamed(fp_out, yield_zmwtuple_func, zmw_filter_func)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 140, in write_streamed
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 357, in
    main(sys.argv) # pragma: no cover
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 354, in main
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 267, in cmd_run_streamed_median_filter
    run_streamed_median_filter(fp_in, sys.stdout, whitelist_set)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 147, in run_streamed_median_filter
    write_streamed(fp_out, yield_zmwtuple_func, zmw_filter_func)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 140, in write_streamed
    fp_out.write(str(zrec.subread_record))
    fp_out.write(str(zrec.subread_record))
    IOError: [Errno 32] Broken pipe
    IOError: [Errno 32] Broken pipe
    INFO:root: --by-strand SNV consensus errors #442 count= 449,237
    cat: write error: Broken pipe
    cat: write error: Broken pipe
    [WARNING]Call 'bash -vex build_db.sh' returned 256.
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    [WARNING]Call 'bash -vex build_db.sh' returned 256.
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1443, in
    main()
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1439, in main
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1443, in
    main()
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1439, in main
    args.func(args)
    INFO:root:Counting bytes from "None"
    INFO:root: Update issue templates #1 count= 201
    INFO:root: Consistency and FAQs #2 count= 422
    INFO:root: Add license #4 count= 884
    INFO:root: isoseq3 package not found at bioconda #8 count= 1,773
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 985, in cmd_build
    build_db(ours, args.input_fofn_fn, args.db_fn, args.length_cutoff_fn)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 167, in build_db
    io.syscall('bash -vex {}'.format(script_fn))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 985, in cmd_build
    INFO:root: blasr bam output problem #15 count= 3,383
    INFO:root: Updating metadata for pbbam package #29 count= 6,612
    build_db(ours, args.input_fofn_fn, args.db_fn, args.length_cutoff_fn)
    INFO:root: Falcon: parameters to optimize haplotype resolution #57 count= 13,179
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 167, in build_db
    INFO:root: Iso-Seq 3 cluster fails with segfault in clustering #61 count= 37,191
    io.syscall('bash -vex {}'.format(script_fn))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 357, in
    INFO:root:Counting bytes from "None"
    main(sys.argv) # pragma: no cover
    INFO:root: Update issue templates #1 count= 201
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 354, in main
    INFO:root: Consistency and FAQs #2 count= 422
    args.func(args)
    INFO:root: Add license #4 count= 884
    INFO:root: isoseq3 package not found at bioconda #8 count= 1,773
    INFO:root: pbfusion error #657 count= 861,953
    INFO:root: blasr bam output problem #15 count= 3,383
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 267, in cmd_run_streamed_median_filter
    run_streamed_median_filter(fp_in, sys.stdout, whitelist_set)
    INFO:root: Updating metadata for pbbam package #29 count= 6,612
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 147, in run_streamed_median_filter
    write_streamed(fp_out, yield_zmwtuple_func, zmw_filter_func)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 140, in write_streamed
    fp_out.write(str(zrec.subread_record))
    IOError: [Errno 32] Broken pipe
    INFO:root: Falcon: parameters to optimize haplotype resolution #57 count= 13,179
    INFO:root: Iso-Seq 3 cluster fails with segfault in clustering #61 count= 37,191
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 357, in
    main(sys.argv) # pragma: no cover
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 354, in main
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 267, in cmd_run_streamed_median_filter
    run_streamed_median_filter(fp_in, sys.stdout, whitelist_set)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 147, in run_streamed_median_filter
    write_streamed(fp_out, yield_zmwtuple_func, zmw_filter_func)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/fasta_filter.py", line 140, in write_streamed
    fp_out.write(str(zrec.subread_record))
    IOError: [Errno 32] Broken pipe
    cat: write error: Broken pipe
    [WARNING]Call 'bash -vex build_db.sh' returned 256.
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    raise Exception(msg)
    Exception: Call 'bash -vex build_db.sh' returned 256.
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1443, in
    INFO:root: #890 count= 1,685,294
    raise Exception(msg)
    Exception: Call 'bash -vex build_db.sh' returned 256.
    cat: write error: Broken pipe
    [WARNING]Call 'bash -vex build_db.sh' returned 256.
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1443, in
    main()
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1439, in main
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 985, in cmd_build
    build_db(ours, args.input_fofn_fn, args.db_fn, args.length_cutoff_fn)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 167, in build_db
    io.syscall('bash -vex {}'.format(script_fn))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)
    Exception: Call 'bash -vex build_db.sh' returned 256.
    main()
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 1439, in main
    args.func(args)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 985, in cmd_build
    build_db(ours, args.input_fofn_fn, args.db_fn, args.length_cutoff_fn)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/falcon_kit/mains/dazzler.py", line 167, in build_db
    io.syscall('bash -vex {}'.format(script_fn))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)
    Exception: Call 'bash -vex build_db.sh' returned 256.
    2019-03-14 17:52:53,047 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,047 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,047 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,047 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,048 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,048 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,048 - root - CRITICAL - Error in /pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    2019-03-14 17:52:53,049 - root - CRITICAL - Error in /pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    "main", fname, loader, pkg_name)
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in
    main()
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 259, in main
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in
    main()
    run(**vars(parsed_args))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 253, in run
    run_cfg_in_tmpdir(cfg, tmpdir, '.')
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 259, in main
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 228, in run_cfg_in_tmpdir
    run_bash(bash_template, myinputs, myoutputs, parameters)
    run(**vars(parsed_args))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 253, in run
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 187, in run_bash
    util.system(cmd)
    run_cfg_in_tmpdir(cfg, tmpdir, '.')
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 228, in run_cfg_in_tmpdir
    raise Exception(msg)
    Exception: Call '/bin/bash user_script.sh' returned 256.
    run_bash(bash_template, myinputs, myoutputs, parameters)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 187, in run_bash
    util.system(cmd)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)
    Exception: Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,055 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,056 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,056 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,056 - root - CRITICAL - Error in /pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in
    main()
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 259, in main
    run(**vars(parsed_args))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 253, in run
    run_cfg_in_tmpdir(cfg, tmpdir, '.')
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 228, in run_cfg_in_tmpdir
    run_bash(bash_template, myinputs, myoutputs, parameters)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 187, in run_bash
    util.system(cmd)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)
    Exception: Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,065 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
    2019-03-14 17:52:53,066 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,066 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 17:52:53,067 - root - CRITICAL - Error in /pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/pylon5/mc5fqip/orca21/0-rawreads/build/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
    Traceback (most recent call last):
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 174, in _run_module_as_main
    "main", fname, loader, pkg_name)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/runpy.py", line 72, in _run_code
    exec code in run_globals
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in
    main()
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 259, in main
    run(**vars(parsed_args))
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 253, in run
    run_cfg_in_tmpdir(cfg, tmpdir, '.')
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 228, in run_cfg_in_tmpdir
    run_bash(bash_template, myinputs, myoutputs, parameters)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/do_task.py", line 187, in run_bash
    util.system(cmd)
    File "/pylon5/mc5fqip/orca21/anaconda3/envs/myenv/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
    raise Exception(msg)
    +++ pwd
    ++ echo 'FAILURE. Running top in /pylon5/mc5fqip/orca21/0-rawreads/build (If you see -terminal database is inaccessible- you are using the python bin-wrapper, so you will not get diagnostic info. No big deal. This process is crashing anyway.)'
    ++ rm -f top.txt
    Exception: Call '/bin/bash user_script.sh' returned 256.
    +++ pwd
    ++ echo 'FAILURE. Running top in /pylon5/mc5fqip/orca21/0-rawreads/build (If you see -terminal database is inaccessible- you are using the python bin-wrapper, so you will not get diagnostic info. No big deal. This process is crashing anyway.)'
    ++ rm -f top.txt
    +++ pwd
    ++ echo 'FAILURE. Running top in /pylon5/mc5fqip/orca21/0-rawreads/build (If you see -terminal database is inaccessible- you are using the python bin-wrapper, so you will not get diagnostic info. No big deal. This process is crashing anyway.)'
    ++ rm -f top.txt
    +++ pwd
    ++ echo 'FAILURE. Running top in /pylon5/mc5fqip/orca21/0-rawreads/build (If you see -terminal database is inaccessible- you are using the python bin-wrapper, so you will not get diagnostic info. No big deal. This process is crashing anyway.)'
    ++ rm -f top.txt
    INFO:root: Update issue templates #1,937 count= 3,336,834
    ++ which python
    ++ which python
    ++ which python
    ++ which python
    ++ which top
    ++ which top
    ++ which top
    ++ which top
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    ++ env -u LD_LIBRARY_PATH top -b -n 1
    INFO:root: Consistency and FAQs #2,595 count= 6,653,207
    ++ pstree -apl
    ++ pstree -apl
    ++ pstree -apl
    ++ pstree -apl

real 4m44.057s
user 4m5.866s
sys 0m27.214s

real 4m44.060s
user 4m5.665s
sys 0m27.575s

real 4m44.080s
user 4m6.569s
sys 0m26.420s

  • finish
  • echo 'finish code: 1'

real 4m44.091s
user 4m7.281s
sys 0m26.221s

DBsplit -f -a -x500 -s200 raw_reads

  • DBsplit -f -a -x500 -s200 raw_reads
    #LB=$(cat raw_reads.db | LD_LIBRARY_PATH= awk '$1 == "blocks" {print $3}')
    #echo -n $LB >| db_block_count

CUTOFF=$(python2.7 -m falcon_kit.mains.calc_cutoff --coverage 30.0 1100000000 <(DBstats -b1 raw_reads))
++ python2.7 -m falcon_kit.mains.calc_cutoff --coverage 30.0 1100000000 /dev/fd/63
+++ DBstats -b1 raw_reads
falcon-kit 1.2.6
pypeflow 2.1.1

  • CUTOFF=13068
    echo -n $CUTOFF >| length_cutoff
  • echo -n 13068

TODO: Verify that db exists.

#ln -sf length_cutoff length_cutoff

date

  • date
    2019-03-14 19:11:47,694 - root - DEBUG - Call '/bin/bash user_script.sh' returned 0.
    2019-03-14 19:11:47,694 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'
    2019-03-14 19:11:47,695 - root - DEBUG - Checking existence of u'length_cutoff' with timeout=30
    2019-03-14 19:11:47,739 - root - DEBUG - Checking existence of u'raw_reads.db' with timeout=30
    2019-03-14 19:11:47,739 - root - WARNING - CD: '/pylon5/mc5fqip/orca21/0-rawreads/build' -> '/pylon5/mc5fqip/orca21/0-rawreads/build'

real 83m38.191s
user 92m3.326s
sys 6m48.399s
touch /pylon5/mc5fqip/orca21/0-rawreads/build/run.sh.done

  • touch /pylon5/mc5fqip/orca21/0-rawreads/build/run.sh.done
  • finish
  • echo 'finish code: 0'

@pb-cdunn
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  1. Did you cross-post this? It looks a lot like lfs setstripe -c 12 ? FALCON#588
  2. If you're going to use GitHub, you need to learn to use GitHub. You can "attach" a file, or you can post it somewhere (e.g. gist.github.com), or you can post it here with markdown formatting if it's small. Other users are able to figure this out. You're just dumping a lot of text.
  3. You need to debug this COMPLETELY OUTSIDE THE WORKFLOW. (See my response to the cross-post.) The bug appears to be specific to your hardware. You should be able to run any task yourself, independent of the workflow, and to learn how much memory it consumes.

We interact with GitHub so we can learn about small bugs directly from users, but this is a free service. Management does not give us much bandwidth to respond to GitHub tickets. Please do everything you can to reduce the time we need to spend. If you prove that we have a bug, we will try to fix it quickly.

If you are running Sequel and using official PB "smrtlink" software, you can get more support through your PacBio representatives.

@NicMAlexandre
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NicMAlexandre commented Mar 26, 2019 via email

@xiaoyezao
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Hi nicolasalexandre21,

Have you solved your problems?

I got similar issues with slurm, but sloved by addiing the options : --ntasks 1 --exclusive in srun command. I don't know why, but it works (I learned this when playing with canu). It seems that without --ntasks 1 , thesrun will repeat the job many times simultaneously which causes chaos to the processing.

Let me know whether this helps.

@pb-cdunn
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Thanks. I've updated the pypeflow wiki with that helpful advice.

@NicMAlexandre
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NicMAlexandre commented Mar 29, 2019 via email

@NicMAlexandre
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NicMAlexandre commented Apr 11, 2019 via email

@pb-cdunn
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I should have mentioned: Always start with a tiny testcase. Integration problems are the worst!

Glad you got it working.

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